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This is a photomicrograph of a section of skin
from a frog with chytridiomycosis. B. dendrobatidis
are the roundish structures in the stratum corneum (the top
layer of the epidermis). Some are empty and often become flattened,
but others contain
internal structures. Compare what you can see to the diagrams in
the previous slide. Zoospores are marked with an arrow and "Z".
A discharge papilla can be seen deeper in the stratum corneum. An arrow with "T" marks this. The epithelial cells of the host are affected by the presence of B. dendrobatidis and respond by increasing in number. In a normal frog skin the stratum corneum is a layer about 2-3 cells thick. In this specimen the stratum corneum is multilayered and disorganised. E = epidermis, D = dermis. |