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Long-read Sequencing for Research in the Tropics : Round 4

The Centre for Tropical Bioinformatics and Molecular Biology is once again offering its members the chance to win access to free long read sequencing using an Oxford Nanopore sequencer. This time the centre has four MinION flow cells and reagents for standard ligation library prep.

Nanopore sequencing is a type of single molecule nucleic acid (DNA or RNA) sequencing that offers ultra-long reads, rapid operation and relatively high total throughput. Oxford Nanopore develops a range of instruments for nanopore sequencing including the MinION, which is highly portable (phone sized), inexpensive and flexible -making it particularly suited to molecular biology in the tropics.

CTBMB has four MinION flow cells as well as basic reagents for preparing DNA sequencing libraries. These will be used to support small sequencing projects using the MinION. Applications are encouraged from all CTBMB members, however, you will need to be relatively self-sufficient with the associated labwork. If you are unsure whether the system is appropriate for your research you should first explore examples listed here or seek advice from

Non-members can submit a membership application online

For more information on the application and submission form please download the following

Grant Details

Application Template

Submission and assessment process;

Grant applications should be a single page or less. Submit your application by email to no later than 15th March 2024.

Applications will be assessed by the CTBMB executive team and applicants will be notified of the outcome by 1st April 2024.

Past Grants

Successful Projects

  • Dr Kelly Condon: Unravelling the Vibrio spp. soup associated with mass mortality in an Australian prawn hatchery.
  • Dr Bruna Luz: Whole genome of Tubastrea coccinea
  • Paul O'Brien: Investigating the sponge metagenome using long-read sequencing
  • Erika Gress: First high-quality transcriptome of a Black Coral (Antipatharia)

Single-cell Grant Description  (PDF, 284 KB)

Successful Projects

  • Dr Margaret Jordan and Ms Annie Willson: EOMES role in MS pathogenesis is via monocyte-mediated cytotoxicity
  • Dr Andreas Kupz: Investigating the impact of novel live attenuated tuberculosis vaccines on trained immunity in lung immune cells
  • Dr Martha Cooper, Jamie Brady and Prof. Denise Doolan: Pilot study to assess heterogeneity of the human NK cell response to experimental P. falciparum malaria.
  • Dr Bruna Luz: Single-cell approach in Scleractinia
  • Dr Roland Ruscher: Molecular pathways underlying the anti-inflammatory effects of hookworm-secreted proteins in human Inflammatory Bowel Disease.

Successful Projects

  • Madalyn Cooper and Alyssa Budd: Nanopore genome skimming of the critically endangered largetooth sawfish
  • Sandra Infante-Villamil, Dr Roger Huerlimann, Dr Kelly Condon, Prof. Dean Jerry: Characterisation of low complexity but potentially high-risk bacterial communities in early developmental stages of Black tiger prawn hatcheries
  • Dr Jenny Elliman: Aquatic invertebrate disease causing organisms
  • Dr Alanna Sorenson: De novo genome assembly of KRX E. coli strain for crucial in vivo bacterial replication termination data
  • Dr Peter Cowman: Surviving 50 million years on tropical reefs – a genome for Zanclus cornutus, a monotypic reef fish
  • Dr Paul O’Brien, A. Prof. David Bourne, Dr. Nicole Webster: Host-Microbe coevolution in coral reef invertebrates

Successful Project

  • Ryley Dorney: Paving the way for personalised medicine with long-read transcriptomics in treatment of liver cancer